The multidisciplinary research in the Peng group aims to elucidate the molecular mechanisms by which human diseases occur. We develop and integrate a diverse toolbox spanning single-molecule microscopy, super-resolution microscopy, spectroscopy, nanomaterial engineering, biophysics, chemical biology, and quantitative modeling to uncover previously unexplored biological processes. With bright and photostable probes, we have unprecedented capability to record ultralong-term “molecular movies” in living systems with high spatiotemporal resolutions and reveal molecular interactions that drive biological functions.